Philadelphia University + Thomas Jefferson University

Publications

Honda, S., Loher, P., Shigematsu, M., Palazzo, J.P., Suzuki, R., Imoto, I., Rigoutsos, I. and Kirino, Y. Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers. Proceedings of the National Academy of Sciences, USA, 112: E3816-E3825, 2015.

We discovered that a novel type of tRNA-derived small RNA, termed SHOT-RNAs, are specifically and abundantly expressed in hormonally-driven breast and prostate cancers and enhance cell proliferation


Honda, S., Morichika, K. and Kirino, Y. Selective amplification and sequencing of cyclic phosphate-containing RNAs by the cP-RNA-seq method. Nature Protocols, 11: 476-489, 2016.

We developed a “cP-RNA-seq” method that can exclusively amplify and sequence cyclic phosphate (cP)-containing RNAs, such as 5′-tRNA halves.


Honda, S. and Kirino, Y. Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences. Nucleic Acids Research. pii: gkv218., 2015.

We developed “Dumbbell PCR (Db-PCR)”, an efficient and convenient method to distinctively quantify a specific individual small RNA variant.


Honda, S., Shigematsu, M., Morichika, K., Telonis, A.G. and Kirino, Y. Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature transfer RNA. RNA Biology 12: 501-508, 2015.

We developed “Four-Leaf clover qRT-PCR (FL-PCR)”, an efficient and convenient method to specifically quantify mature tRNAs.


Honda, S., Kirino, Y., Maragkakis, M., Alexiou, P., Ohtaki, A., Murali, R., Mourelatos, Z. and Kirino, Y.  Mitochondrial protein BmPAPI modulates the length of mature piRNAs. RNA 19: 1-14, 2013.

We identified BmPAPI as a novel piRNA biogenesis factor helping piRNA precursor processing on mitochondrial outer membrane.  


Recent Publications