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Photoactive Dye Could Prevent Infection During Bone-repair Surgery

05/06/15

A green dye that sticks to bone grafts becomes antimicrobial with the flick of a light switch and could help reduce the risk of infections during bone-reconstruction surgeries.

(PHILADELPHIA) – Despite extensive procedures to sterilize small and large bone fragments used in joint replacement or reconstructive surgeries, the rate of infection remains around 5 percent and can reach 11 percent or even higher in bone repairs for gunshot wounds or reconstruction after tumor removal. Infection after surgery is a serious complication that can require further surgery and can be life threatening. A new study demonstrates for the first time that an antimicrobial dye activated by light avidly adheres to bone to prevent bacteria from growing on bone fragments used in reconstructive surgery, and remove any bacteria that has already attached, thereby sterilizing the bone for surgery. The study was published online April 17 ahead of print in the journal of Clinical Orthopaedics and Related Research.

“We used a class of chemicals called porphyrins that are tolerated very well by the body in the dark and appear to have excellent antimicrobial properties in the presence of light,” says Noreen Hickok, Ph.D., Associate Professor of Orthopedic Surgery at Thomas Jefferson University. “These properties allow sterilization during surgical procedures, which occur in bright light.”

Surgeons often use bone chips or bone powder as a sort of putty during bone reconstruction to help areas of bone re-grow. Also, larger pieces of bone are used all over the world when a tumor or accident requires replacement of a large segment of bone. These bone materials can come either from the patient or a donor and are typically sterilized with a series of methods including various detergents and high pressure steam sterilization. But bacteria can still creep in once the material is handled again. “Bacteria really love to hide and grow in the nooks and crannies of porous bone and bone fragments – it’s one of the most perfect surfaces for bacterial growth,” says Dr. Hickok.

The researchers took these bone chips and treated them with a green dye called TAPP (which stands for 5,10,15,20-tetrakis-(4-aminophenyl)-porphyrin). They showed that in the dark, TAPP is stable. But when the lights go on, TAPP becomes active, producing chemicals called reactive oxygen species, or ROS, that rapidly kill the bacteria. Dr. Hickok and colleagues first treated the bone putty with TAPP and then exposed those fragments to bacteria. As long as the lights were on, bacteria was unable to attach and grow on the surface of the bone.

The researchers then showed that TAPP not only prevents bacterial growth, but can also break up bacterial slime, or biofilms, already growing on the bone fragments. They demonstrated this by first allowing bacteria to colonize the bone and then treating with TAPP. Finally, Dr. Hickok and colleagues showed that the dye binds tightly to the bone, without any trace of leaching out into surrounding fluid, suggesting that it could be safe and non-toxic to normal tissue once implanted.

In theory, says Dr. Hickok, the TAPP dye could be added to the currently used protocols for sterilizing the bone prior to use in surgery. “Sterilization could then occur in two steps—one which was achieved with a targeted illumination, and the other would be the continuation of the activation in the bright lights of the surgical suite so that the sterilizing effects of the ROS release could continue well into surgery and implantation,” says Dr. Hickok. “We need to continue testing in conditions that more closely resemble the surgical suite, but we think that this method could offer a more effective method to help improve patient outcomes by reducing infection rates.”

This research was supported by a National Institute of Arthritis and Musculoskeletal and Skin Diseases training grant T32-AR-052273, a National Institutes of Health grant R01 HD061053, research funding from the National Institutes of Health, Synergy Biomedical, Collegeville, PA, USA, and Zimmer, Warsaw, IN, USA. The Musculoskeletal Transplant Foundation provided human bone allograft.

For more information, contact Edyta Zielinska, 215-955-5291, edyta.zielinska@jefferson.edu.

About Jefferson — Health is all we do.

Our newly formed organization, Jefferson, encompasses Jefferson Health and Thomas Jefferson University, representing our clinical and academic entities.  Together, the people of Jefferson, 19,000 strong, provide the highest-quality, compassionate clinical care for patients, educate the health professionals of tomorrow, and discover new treatments and therapies that will define the future of health care.

Jefferson Health comprises five hospitals, 13 outpatient and urgent care centers, as well as physician practices and everywhere we deliver care throughout the city and suburbs across Philadelphia, Montgomery and Bucks Counties in Pa., and Camden County in New Jersey.  Together, these facilities serve more than 78,000 inpatients, 238,000 emergency patients and 1.7 million outpatient visits annually.  Thomas Jefferson University Hospital is the largest freestanding academic medical center in Philadelphia.  Abington Hospital is the largest community teaching hospital in Montgomery or Bucks counties.  Other hospitals include Jefferson Hospital for Neuroscience in Center City Philadelphia; Methodist Hospital in South Philadelphia; and Abington-Lansdale Hospital in Hatfield Township.

Thomas Jefferson University enrolls more than 3,900 future physicians, scientists, nurses and healthcare professionals in the Sidney Kimmel Medical College (SKMC); Jefferson Schools of Health Professions, Nursing, Pharmacy, Population Health; and the Graduate School of Biomedical Sciences, and is home of the National Cancer Institute (NCI)-designated Sidney Kimmel Cancer Center

For more information and a complete listing of Jefferson services and locations, visit www.jefferson.edu.