Philadelphia University + Thomas Jefferson University

Kirino, Yohei

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Yohei Kirino, PhD

Contact Dr. Kirino

1020 Locust Street
JAH Suite #M-81
Philadelphia, PA 19107

(215) 503-8648
(215) 955-5058 fax

Research Interests:

Small regulatory RNAs have emerged recently as one of the most novel and exciting areas of gene expression regulation. Three major classes of small regulatory RNAs have been identified so far: microRNAs (miRNAs), short-interfering RNAs (siRNAs) and PIWI-interacting RNAs (piRNAs). Our overall research goal is to understand biogenesis and molecular function of the small regulatory RNAs, and use the knowledge to develop a novel therapeutic application in human diseases.

The Kirino lab is particularly focused on piRNAs, the most recently discovered small regulatory RNAs. piRNAs are 24-31 nucleotides and interact with PIWI proteins to play essential roles in germline development. We are currently taking advantage of mouse, Drosophila and Bombyx systems to clarify the biogenesis and function of piRNAs, and to uncover the molecular mechanism of germline development regulated by piRNAs. Since recent studies have shown that PIWI proteins are aberrantly expressed in a variety of cancers and their expression correlates with clinical prognosis, the research project will impact biomedical goals of understanding and conquering cancers as well as reproductive system diseases. We are also performing tRNA researches to develop novel biomarkers and therapeutic applications for cancers and mitochondrial diseases.


Most Recent Peer-Reviewed Publications

  1. Dumbbell-PCR for discriminative quantification of a small RNA variant
  2. MINTbase v2.0: A comprehensive database for tRNA-derived fragments that includes nuclear and mitochondrial fragments from all the Cancer Genome Atlas projects
  3. Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells
  4. The biogenesis pathway of tRNA-derived piRNAs in Bombyx germ cells
  5. YAMAT-seq: An efficient method for high-throughput sequencing of mature transfer RNAs
  6. 5′-Terminal nucleotide variations in human cytoplasmic tRNAHisGUGand its 5′-halves
  7. Morphological and histomorphological structures of testes and ovaries in early developmental stages of the silkworm, Bombyx mori
  8. Consequential considerations when mapping tRNA fragments
  9. Selective amplification and sequencing of cyclic phosphate-containing RNAs by the cP-RNA-seq method
  10. Identification and Functional Analysis of the Pre-piRNA 3′ Trimmer in Silkworms
  11. Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers
  12. tRNA-derived short non-coding RNA as interacting partners of argonaute proteins
  13. Analysis of 13 cell types reveals evidence for the expression of numerous novel primate- And tissue-specific microRNAs
  14. Dumbbell-PCR: A method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences
  15. Mitochondrial tRNA-lookalikes in nuclear chromosomes: Could they be functional?
  16. Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA
  17. Dissecting tRNA-derived fragment complexities using personalized transcriptomes reveals novel fragment classes and unexpected dependencies
  18. Nuclear and mitochondrial tRNA-lookalikes in the human genome
  19. Analysis of sDMA modifications of PIWI proteins
  20. Mitochondrial protein BmPAPI modulates the length of mature piRNAs