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DIRECT DNA SEQUENCING of the MSH2 and MLH1 GENES |
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Germline
DNA is isolated from whole blood. The
exons, including intron-exon boundaries are amplified by PCR and the PCR
products are sequenced in both directions.
The sequences are compared to the published sequences for the respective
genes. Sequence alterations are
compared to published polymorphisms and mutations. However, large genomic deletions can not be assessed with
this technology. |
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