The Sidney Kimmel Cancer Center Flow Cytometry Shared Resource (FCSR) provides state-of-the-art fluorescence activated cell sorting and analysis capabilities to investigators of the Sidney Kimmel Cancer Center and the Philadelphia region. The most commonly used applications are multi-color cell surface phenotyping, detection of intracellular cytokines and signaling molecules, transfection measurements, studies of apoptosis, cell cycle analysis, cell proliferation, and real time analysis of Ca2+ mobilization kinetics. The sorters, analyzers, and workstations allow for affordable, reliable, and accurate characterization of a wide variety of biological samples. The services provided by the facility can be tailored to suit experimental designs in a variety of fields. FCSR provides consultation on current protocols, analysis techniques, and data presentation. This resource plays a critical role in assisting SKCC members performing various research projects directly related to the understanding of the biology and treatment of cancer. Current goals of the facility include:
- Provide high-quality and affordably priced services for analytical cytometry data acquisition and cytometric cell sorting of various types of biological particles.
- Consult with SKCC members to determine analytical cytometry needs, aid in the development of appropriate protocols and techniques, and provide technical support for analysis including data analysis and interpretation and/or training in using BD FACS DIVA and FlowJo softwares.
- Provide training to SKCC members for 24/7 use of automated analytical flow cytometry instrumentation for competent and efficient routine analysis.
Jianke Zhang PhD, Associate Professor of Microbiology and Immunology, leads this Shared Resource and has done so for the past 11 years. The SR Manager, Lei Yu PhD, oversees day-to-day operations.
The Flow Cytometry Shared Resource is centrally located in the Bluemle Life Sciences Building and after training, is accessible for experienced, trained investigators 24 hours a day, seven days a week through the iLab scheduling system.